A lysine residue essential for geminivirus replication also controls nuclear localization of the TYLCV Rep protein. [Virus-Cell Interactions]

Geminiviruses are ssDNA viruses that infect an extensive range of plants. To promote viral replication, geminiviruses control the host cell cycle. The viral protein Rep is crucial to reprogram the cell cycle and then initiate viral DNA replication by interacting with an array of atomic host facets. Even though many protein domains of Rep have been characterized, little is understood concerning its own nuclear targeting. Here, we show that one conserved lysine in the N-terminal portion of Rep is pivotal for nuclear localization of the Rep protein out of Tomato Yellow Leaf Curl Virus (TYLCV) with two other lysines also causing its nuclear import. We show that mutating these lysines contributes to atomic exception of TYLCV Rep without undermining its interaction with all SCE1. Furthermore, the capability of TYLCV Rep to promote viral DNA replication additionally depends upon this exceptionally conserved lysine independently of its position in export of Rep. Our data thus demonstrate that this lysine potentially includes a broad function in geminivirus replication, however its role in nuclear import and SCE1-binding differs depending upon the Rep protein examined.
Importance
We define this a exceptionally conserved lysine is essential for atomic of Rep in three distinct begomoviruses. To your knowledge, it may be the very first time that nuclear localization is well known for virtually any geminiviral Rep protein. Our data adds still yet another key function for the lysine residue, moreover its functions in viral DNA replication and interaction with host factors, like the SUMO E2 conjugating enzyme.

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