Serial Section Array Scanning Electron Microscopy Analysis of Cells from Lung Autopsy Specimens Following Fatal A/H1N1 2009 Pandemic Influenza Virus Infection [Virus-Cell Interactions]

IMPORTANCE Ordinarily, it’s tough to see IAV particles in post-mortem samples from patients with seasonal influenza. Actually, only a few viral antigens are present in bronchial epithelial cells in autopsied lung parts. Previously, we discovered many viral antigens in AEC-IIs from the lungdisease. This was since nearly all A/H1N1/pdm09 in the lung tissue uttered an aspartic acid to glycine substitution at position 222 (D222G) of their hemagglutinin protein. A/H1N1/pdm09 harboring the D222G substitution comes with a receptor-binding preference alpha;-2,3-linked sialic acids extracted on individual AECs and infects them at precisely exactly the same way as H5N1 and H7N9 avian IAVs. We examine the first successful observation of virus contamination not only in AEC-IIs, but also in Ms/Ms and Neus, together with electron microscopy. The finding of a M/M harboring numerous virus particles within vesicles and at the surface suggests that Ms/Ms take part in the pathogenesis of all IAV primary pneumonia.
A/H1N1 2009 pandemic flu virus (A/H1N1/pdm09) was first identified as a book pandemic influenza A virus (IAV) in 2009. Previously, we reported that many viral antigens were detected in type II alveolar epithelial tissues (AEC-IIs) within autopsied lung tissue in a patient using A/H1N1/pdm09 pneumonia. To analyze the supply of virus particles and morphological alterations in host cells, then the human autopsied lung specimens from this patient were analyzed using transmission electron microscopy (TEM) and also a publication scanning electron microscopy (SEM) procedure. We identified virus particles and intranuclear dense tubules, which are connected with matrix 1 (M1) proteins from IAV. Large scale twodimensional observation was enabled by ‘stitching’ together contiguous SEM images. A single whole cell analysis with a sequential section array (SSA)-SEM identified virus contamination from vesicles within the cytoplasm and/or around the cell surface of AEC-IIs, Ms/Ms, and Neus; however, intranuclear dense tubules were found just in AEC-IIs. Computer-assisted processing of SSA-SEM images from each celltype allowed 3D modeling of the supply of virus particles within an ACE-II, also a M/M, and also a Neu.

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