The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus induces STAT2 degradation to inhibit interferon signaling [Pathogenesis and Immunity]

Interferons (IFNs) play a crucial role in host antiviral response via activating JAK/STAT (Janus kinase/signal transducer and activator of transcription) signaling pathway to induce expression of myriad genes. STAT2 is a key player in the IFN-activated JAK/STAT signaling. Porcine reproductive and respiratory syndrome virus (PRRSV) is an important viral pathogen causing huge loss to the swine industry. PRRSV infection elicits a meager protective immune response in pigs. The objective of this study was to investigate the effect of PRRSV on STAT2 signaling. Here, we demonstrated that PRRSV downregulated STAT2 to inhibit IFN-activated signaling. PRRSV strains of both PRRSV-1 and PRRSV-2 species reduced STAT2 protein level, whereas the STAT2 transcript level had minimal change. PRRSV reduced the STAT2 level in a dose-dependent manner and shortened STAT2 half-life significantly from approximately 30 to 5 hours. PRRSV-induced STAT2 degradation could be restored by treatment with the proteasome inhibitor MG132 and lactacystin. In addition, PRRSV non-structural protein 11 (nsp11) was identified to interact with and reduce STAT2. The N-terminal domain (NTD) of nsp11 was responsible for STAT2 degradation and interacted with STAT2 NTD and coil-coil domain (CCD). Mutagenesis analysis showed that the amino acid residue K59 of nsp11 was indispensable for inducing STAT2 reduction. Mutant PRRSV with the K59A mutation generated by reverse genetics almost lost the ability to reduce STAT2. Together, these results demonstrate that PRRSV nsp11 antagonizes IFN signaling via mediating STAT2 degradation and provide further insights into the PRRSV interference of the innate immunity.

SIGNIFICANCE PRRSV infection elicits a meager protective immune response in pigs. One of the possible reasons is that PRRSV antagonizes interferon induction and its downstream signaling. Interferons are key components in the innate immunity and play crucial roles against viral infection and in the activation of adaptive immune response via JAK/STAT signaling. STAT2 is indispensable in the JAK/STAT signaling as it is also involved in activation of antiviral activity in the absence of STAT1. Here, we discovered that PRRSV nsp11 downregulates STAT2. Interestingly, the N-terminal domain of nsp11 is responsible for inducing STAT2 degradation and directly interacts with STAT2 N-terminal domain. We also identified a crucial amino acid residue K59 in nsp11 as a mutation of it led to the loss of the capability to downregulate STAT2. A mutant PRRSV with mutation of K59 had minimal effect on STAT2 reduction. Our data provide further insights into PRRSV interference with interferon signaling.

DNA packaging and genomics of the Salmonella 9NA-like phages [Genetic Diversity and Evolution]

We present the genome sequences of Salmonella enterica tailed phages Sasha, Sergei, and Solent. These phages, along with Salmonella phages 9NA, FSL_SP-062 and FSL_SP-069 and the more distantly-related Proteus phage PmiS-Isfahan have similar sized genomes between 52 and 57 kbp in length that are largely syntenic. Their genomes also show substantial genome mosaicism relative to one another, which is common within tailed phage clusters. Their gene content ranges from 80 to 99 predicted genes, of which 40 are common to all seven and form the core genome which includes all identifiable virion assembly and DNA replication genes. The total number of gene types (pangenome) in the seven phages is 176, and 59 of these are unique to individual phages. Their core genomes are much more closely related to one another than to any other known phage, and they comprise a well-defined cluster within the family Siphoviridae. To begin to characterize this group of phages in more experimental detail, we identified the genes that encode the major virion proteins and examined the DNA packaging of the prototypic member, phage 9NA. We showed that it uses a pac site-directed headful packaging mechanism that results in virion chromosomes that are circularly permuted and about 13% terminally redundant. We also showed that its packaging series initiate with dsDNA cleavages that are scattered across a 170 bp region, and that its headful measuring device has a precision of ±1.8%.

IMPORTANCE The 9NA-like phages are clearly highly related to each other but are not closely related to any other known phage type. This work describes the genomes of three new 9NA-like phages, and experimental analysis of the proteome of the 9NA virion and DNA packaging into the 9NA phage head. There is increasing interest in the biology of phages because of their potential for use as antibacterial agents and for their ecological roles in bacterial communities. 9NA-like phages have been identified that infect two bacterial genera to date and related phages infecting additional Gram-negative hosts are likely to be found in the future. This work provides a foundation for the study of these phages which will facilitate their study and potential use.

Read More

Interference with SAMHD1 restores late gene expression of modified vaccinia virus Ankara (MVA) in human dendritic cells and abrogates type I interferon expression. [Virus-Cell Interactions]

Attenuated poxviruses, like the modified vaccinia virus Ankara (MVA), are promising vectors for vaccines against infectious diseases and cancer. However, host innate immune responses interfere with the viral life cycle and also influence the immunogenicity of vaccine vectors. The sterile alpha motif (SAM) domain and histidine-aspartate (HD) domain-containing protein 1 (SAMHD1) is a phosphohydrolase and reduces the cellular deoxynucleoside triphosphate (dNTP) concentration, which impairs poxviral DNA replication in human dendritic cells (DCs). The human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency virus (SIV) encode an accessory protein called viral protein X (Vpx) that promotes proteasomal degradation of SAMHD1, leading to a rapid increase in cellular dNTP concentrations. To study the function of SAMHD1 during MVA infection of human DCs, the SIV vpx gene was introduced into the MVA genome (resulting in recombinant MVA-vpx). Infection of human DCs with MVA-vpx led to SAMHD1 protein degradation and enabled MVA-vpx to replicate its DNA genome and to express genes controlled by late promoters. Late gene expression by MVA-vpx might improve its vaccine vector property however unexpectedly type I interferon expression was blocked by vpx-expressing MVA. MVA-vpx can be used as a tool to study poxvirus host interaction and vector safety.

IMPORTANCE SAMHD1 is a phosphohydrolase and reduces cellular dNTP concentrations, which impairs poxviral DNA replication. The simian SIV accessory protein Vpx promotes degradation of SAMHD1, leading to increased cellular dNTP concentrations. Vpx addition enables poxviral DNA replication in human dendritic cells (DCs) and the expression of viral late proteins, which are normally blocked. SAMHD1 function during modified vaccinia virus Ankara (MVA) infection of human DCs was studied with recombinant MVA-vpx, expressing Vpx. Infection of human DCs with MVA-vpx decreased SAMHD1 protein amounts, enabling MVA DNA replication and expression of late viral genes. Unexpectedly, type I interferon expression was blocked after MVA-vpx infection. MVA-vpx might be a good tool to study SAMHD1 depletion during poxviral infections and provide insights into poxvirus host interaction.

Read More

Validity And Reliability Of The Stages Cycling Power Meter

This study aimed to determine the validity and the reliability of the Stages power meter crank system (Boulder, United States) during several laboratory cycling tasks.
Eleven trained participants completed laboratory cycling trials on an indoor cycle fitted with SRM Professional and Stages systems. The trials consisted of an incremental test at 100W, 200W, 300W, 400W and four 7s sprints. The level of pedaling asymmetry was determined for each cycling intensity during a similar protocol completed on a Lode Excalibur Sport ergometer. The reliability of Stages and SRM power meters was compared by repeating the incremental test during a test-retest protocol on a Cyclus 2 ergometer.
Over power ranges of 100-1250W the Stages system produced trivial to small differences compared to the SRM (standardized typical error values of 0.06, 0.24 and 0.08 for the incremental, sprint and combined trials, respectively). A large correlation was reported between the difference in power output (PO) between the two systems and the level of pedaling asymmetry (r=0.58, p < 0.001). Recalculating PO of the Stages system according to the level of pedaling asymmetry provided only marginal improvements in PO measures. The reliability of the Stages power meter at the sub-maximal intensities was similar to the SRM Professional model (coefficient of variation: 2.1 and 1.3% for Stages and SRM, respectively).
The Stages system is a suitable device for PO measurements, except when a typical error of measurement <3.0% over power ranges of 100-1250W is expected.
Corresponding author: Yann LE MEUR, PhD Laboratoire Motricité Humaine, Education Sport Santé (EA 6312) Faculté des Sciences du Sport, 261 route de Grenoble 06205 NICE Cedex 3 Email: Tel: +33 6 29 40 66 22
Copyright © 2019 by the National Strength & Conditioning Association.

Read More

The Relationship Between Adductor Squeeze Strength, Subjective Markers of Recovery and Training Load in Elite Rugby Players

Tiernan, C, Lyons, M, Comyns, T, Nevill, AM, and Warrington, G. The relationship between adductor squeeze strength, subjective markers of recovery and training load in elite Rugby players. J Strength Cond Res XX(X): 000–000, 2019—The adductor squeeze strength test has become a popular training monitoring marker, particularly in team sports. The aim of this study was to investigate the relationship between adductor squeeze strength scores, subjective markers of recovery and training load in elite Rugby Union players, because of limited research in this area. Nineteen elite male Rugby Union players completed daily monitoring markers (adductor squeeze strength and 5 selected subjective markers of recovery), over a 10-week preseason training period. Rate of perceived exertion (RPE) was collected to determine training load (session RPE; RPE × session duration) and to calculate weekly training load. Spearman’s correlation was used to analyze the relationship between adductor squeeze strength scores, subjective markers of recovery, and weekly training load. The results found that where adductor squeeze scores decreased, both perceived fatigue levels (r = −0.335; R2 = 11.2%; p < 0.001) and muscle soreness (r = −0.277; R2 = 7.7%; p < 0.001) increased. A weak correlation was found between Monday adductor squeeze strength scores and the previous week's training load (r = −0.235; R2 = 5.5%; p < 0.001) and Friday adductor squeeze strength scores and the same week's training load (r = −0.211; R2 = 4.5%; p < 0.05). These results show that adductor squeeze strength may provide coaches with a time-efficient, low‐cost objective, player monitoring marker. Additionally, the combination of adductor strength squeeze, with subjective markers, perceived fatigue, and muscle soreness, and appropriately planned training load may help coaches to optimize training adaptations by determining a player's training status.
Address correspondence to Caoimhe Tiernan,
This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
Copyright © 2019 by the National Strength & Conditioning Association.

Read More

Lnc-MxA inhibits IFN-{beta} transcription via forming RNA-DNA triplexes at its promoter [Pathogenesis and Immunity]

Previously, we identified a set of lncRNAs that were differentially expressed in influenza A virus (IAV)-infected cells. In this study, we focused on lnc-MxA, which is upregulated during IAV infection. We found that the overexpression of lnc-MxA facilitates the replication of IAV, while the knockdown of lnc-MxA inhibits viral replication. Further studies demonstrated that lnc-MxA is an interferon-stimulated gene. However, lnc-MxA inhibits the SeV- and IAV-induced activation of IFN-β. The luciferase assay indicated that lnc-MxA inhibits the activation of the IFN-β reporter upon stimulation with RIG-I, MAVS, TBK1 or active IRF3 (IRF3-5D). These data indicated that lnc-MxA negatively regulates the RIG-I-mediated antiviral immune response. The CHIP assay showed that the enrichment of IRF3 and p65 at the IFN-β promoter in lnc-MxA-overexpressing cells was significantly lower than that in control cells, indicating that lnc-MxA interfered with the binding of IRF3 and p65 to the IFN-β promoter. CHIRP, triplex pulldown and biolayer interferometry assays indicated that lnc-MxA can bind to the IFN-β promoter. Furthermore, the EMSA assay showed that lnc-MxA can form complexes with the IFN-β promoter fragment. These results demonstrated that lnc-MxA can form a triplex with the IFN-β promoter to interfere with the activation of IFN-β transcription. Using a VSV infection assay, we confirmed that lnc-MxA can repress the RLR-mediated antiviral immune response and influence the antiviral status of cells. In conclusion, we revealed that lnc-MxA is an interferon-stimulated gene that negatively regulates the transcription of IFN-β by forming an RNA-DNA triplex.

IMPORTANCE IAV can be recognized as a nonself molecular pattern by host immune systems and can cause immune responses. However, the intense immune response induced by influenza virus known as a “cytokine storm” can also cause widespread tissue damage (Guo XZJ, Thomas PG. 2017. Seminars in Immunopathology 39:1-10; Yokota S. 2003. Nihon Rinsho 61:1953-1958; Clark IA. 2007. Immunology & Cell Biology 85:271-273). Meanwhile, the detailed mechanisms involved in the balancing of immune responses in host cells are not well understood. Our studies reveal that, as an IFN-inducible gene, lnc-MxA functions as a negative regulator of the antiviral immune response. We uncovered the mechanism by which lnc-MxA inhibits the activation of IFN-β transcription. Our findings demonstrate that, as an ISG, lnc-MxA plays an important role in the negative feedback loop involved in maintaining immune homeostasis.

Read More

Book of the Week: Prototyping and Modelmaking for Product Design

Prototyping and Modelmaking for Product Design by Bjarki Hallgrimsson [Source: Amazon]

This week’s selection is “Prototyping and Modelmaking for Product Design” by Bjarki Hallgrimsson.

The process of developing a new product is a series of detailed steps that lead from a general idea to a finished item. Along that long path will be an experimental portion where prototypes are made and tested. How, exactly, is that step done properly? That’s the topic of this book from Bjarki Hallgrimsson, an Associate Professor at the School of Industrial Design at Carleton University in Ottawa.

One might suspect that the process of prototyping involves repeated iterations of a product, and that would be correct, but there is a lot more to the story than simply tweaking a design and rebuilding a prototype.

There are considerable efforts required to work with other people during these iterations to more deeply understand the real problems being solved through the implementation of the design. Typically this is done by exposing a prototype to actual users who can physically evaluate the nature of the product.

And as you might guess, a physical prototype is often built using 3D modeling and 3D printing technologies. That wasn’t always the case, as in the past prototypes were frequently made by hand, due to the cost of setting up manufacturing equipment to make only a single unit.

Hallgrimsson’s book explores the nature of the prototyping and modelmaking processes. Wait, you ask, aren’t those the same thing?

No, they are most definitely not. While Hallgrimsson describes the modelmaking process as the steps required to physically produce the prototype — and he explores a number of non-3D printing methods of doing so — the prototyping part is what he calls a “Design Method”. It’s a process used to study the uses of a proposed product among users by designers.

It’s that non-making process that is of interest to me, as executing that successfully will dramatically increase the probability of devising a successful product. The actual making process, as done by 3D printing, is best learned somewhere other than this book.

That said, Hallgrimsson’s modelmaking section does provide a wealth of advice for the modelmaking process beyond the technology of 3D printing, such as safety, hand tool operation, adhesives, and casting, all of which could be of benefit to Fabbaloo readers.

Hallgrimsson explains the process of prototyping through the exploration of a series of case studies. These case studies include a folding hairdryer, a tablet, an electric vehicle charger, a museum guide and several other seemingly-unrelated objects.

But that’s the point: design is design, regardless of what you’re making. It’s a process you must undertake to arrive at a successful design that provides utility for your users. And, ultimately, your wallet.

If you’re intrigued by the design process, you might check out this book. It’s eminently readable, and you will most definitely learn important things if you do read it.

Via Amazon

Read More

Carbon’s Next DLS Disruption: Specialized 3D Printed Bike Saddle

Specialized Rider Chris Blevins prepares to ride with the new 3D printed S-Works Power Saddle with Mirror Technology [Image: Carbon]

Shoes. Football helmets. Now, Carbon takes its Digital Light Synthesis 3D printing technology to cyclists with a 3D printed bike saddle.

Today the company announces its latest sporty partnership. Carbon is working with California-based Specialized to create “the first digitally printed bike saddle.”

Specialized, which seems to embrace the philosophy of “innovate or die,” has been working to create the best in biking since its founding in 1974. A quick browse through their site shows them to very much cater to serious cyclists; other bike saddles available in their shop retail for $145-$325. Not so much for the casual Sunday cycler, then, but a company that’s really specialized (oh, I get it) in creating advanced solutions.

Bike Saddle Technology


Dust Removed1.jpg

S-Works Power Saddle with Mirror Technology21.jpg

I recall cycling somewhat regularly, if casually, as a teen; I was delighted to learn after some months of my new mountain bike that shops sold gel-style seat pads to make the ride more comfortable. And that was as advanced as I ever got.

What can go into a bicycle seat to make it better? Quite a lot, actually.

Specialized has observed the pressure and discomfort cyclists can undergo due to less-than-optimal seating. These can lead to that tingly sensation to more serious health issues like neuralgia (nerve pain) and urinary problems. Enter science. Specifically, enter digital design.

The newest saddle from Specialized is the S-Works Power Saddle with Mirror Technology. Work with Carbon brings DLS into the picture, as their 3D printing technology enables a lattice design “that enables the saddle to rebound quickly, giving riders the experience of having a ‘suspension’ for their sit bones.”

The shape and structure of the new S-Works Power Saddle disperse the pressure of seating, absorbing impact and improving stability, as well as enhancing breathability of use.

[Image: Carbon]

[Image: Carbon]

EPU material is used in the production of the new seats — the same elastomeric lattice structure and material Carbon has already deployed in high-profile applications with adidas’ FurtureCraft 4D midsole and Riddell’s SpeedFlex Precision-Fit Diamond helmet.

The seat also incorporates Mirror Technology — but the release from Carbon doesn’t detail what that entails, nor is it as yet described on Specialized’s site. We do have a brief mention from the company that also touches on Mirror Technology, but again, not much to go on for now:

“Specialized has a long history of improving the rider experience. We created our first Body Geometry saddle 25 years ago to address serious issues impacting cyclists. Together with Carbon, we are challenging the norms of the cycling world, from design to production time, to create technology that will allow riders to improve performance, increase comfort, and reduce the chance of injury,” said Garrett Gatter, Specialized Product Manager of Saddles. “With Mirror Technology, we’ve only scratched the surface of what’s possible.”

Making The Seat

Pressure mapping the saddle [Image: Carbon]

Pressure mapping the saddle [Image: Carbon]

The S-Works Power Saddle, like its Carbon-powered forerunners, will be produced at scale using the L1 Production Solution introduced early this year.

The announcement notes that “Carbon DLS is helping to bring Mirror Technology to market in record speed.”

Bike saddles on the L1 [Image: Carbon]

Bike saddles on the L1 [Image: Carbon]

Rather than the standard 18-24-month product development timeline, the Carbon/Specialized partnership readied — including more than 70 designed and tested prototypes — the new bike seat in the span of one year. Two months of that (reduced from a standard six months) were dedicated to the overall design process. Design iteration, allocated a standard two-to-three weeks of the process, came down to a single day.

Pricing hasn’t been announced yet for the new seat. It will be available in 2020.

Via Carbon and Specialized

Read More

In-Situ Inspection Gains Momentum In Metal Additive Manufacturing

This briefing chart image from a successfully completed 2017 SBIR Phase I technology project shows the planned setup for LMT [Image: NASA]

As metal additive manufacturing continues to see rising adoption, so too does attention paid to the quality of the parts produced.

It was only a few days ago we heard about a $1.5M grant awarded to Auburn University for advanced metrology for 3D printed parts, and shortly on the heels of that more major funding is set to move forward more advanced inspection.

This time, the US Defense Logistics Agency (DLA) has awarded a $1M contract to a team led by Connecticut-based Flightware.

Layer Topographic Mapping

The grant will extend years-long work Flightware has been leading in the Layer Topographic Mapping (LTM) method. This method, they explain, “determines melt quality directly from as-formed layer surface measurements, unlike other in-situ methods that measure process variables such as temperature to infer as-formed quality anomalies.”

In-situ testing is of rising importance, particularly as applications become more advanced and parts mission-critical, to examine performance in real time. Flightware explains of their LTM work:

“Under the initial effort Flightware demonstrated that the LTM in-situ method can detect melt flaws such as lack of fusion, in real time, on a layer-by-layer basis, with 98.2% detection rate and only 1% false detection rate. Furthermore, after detecting these flaw regions, appropriate repair procedures were automatically defined and then performed, as a demonstration of closed loop control. This essentially eliminated the initial flaw porosity (up to 14%) and the repaired layer was restored to unflawed layer quality (less than 0.2% porosity). This salvaged the part, and allowed acceptance (based on CT scan results) of a part that otherwise would have been scrapped.”

Flightware President Dave Maass adds a bit more, telling us:

“While many in AM are familiar with methods like melt pool monitoring, our approach is fundamentally different because it is based on measurements of the as-formed layer itself, rather than the process conditions used to create the layer.”

Flightware has been awarded several SBIR (Small Business Innovation Research) awards for its LTM method over the last few years, and this latest step will continue this work.

DLA Contract

For this two-year contract, Flightware is working with the Edison Welding Institute (EWI) and Universal Technology Corp. (UTC).

Each plays an important role, as Flightware continues to expand on its work with LTM, EWI develops a “large area profilometry sensor” that can provide precise measurement of the full 3D printer bed, and UTC will see the sensor installed on a Panda system.

We met Panda this year at RAPID + TCT. The large metal 3D printer is from OpenAdditive, UTC’s dedicated additive manufacturing business unit. Designed to be open materials and open architecture, the laser powder bed system is well positioned as a testing base for the in-situ monitoring process.

“…We’re trying to do with laser powder bed fusion metal additive manufacturing what needs to be done,” Ty Pollak, PhD, Director of OpenAdditive, told us when we first saw the Panda.

Now, “what needs to be done” is monitoring. Coupons will be produced on the Panda system to help Flightware validate and improve its algorithms “to reliably determine layer quality of both the as-formed melt and the powder bed.”

Importance Of Inspection

A few terms are common now when discussing metal additive manufacturing; key among them are quality and repeatability.

These technologies are moving rapidly toward production-scale use — but even at lower volumes, quality remains critical. This latest support, from the Department of Defense, highlights the seriousness with which government entities with demanding applications are taking additive manufacturing today.

“DLA is actively applying the benefits of AM to better perform our mission supplying spare parts to the warfighter. Flightware’s in-situ inspection method can reduce scrap and improve yield, which reduces the cost and lead time of parts made by AM methods,” says Denise Price, DLA’s Program Manager for the Small Business Innovation Program.

These oft-touted benefits of 3D printing — reducing cost, weight, waste, and time — mean nothing if the parts produced aren’t up to par. Ensuring, in-situ, that processes are working as intended will help ensure the same for the parts produced.

Via Flightware

Read More

EBM 3D Printing Takes Center Stage With New Center Of Excellence

GE Additive Arcam EBM Center of Excellence in Sweden [Image: GE Additive]

The newest major 3D printing Center of Excellence has opened in Sweden.

GE Additive announced this week the opening of its new Arcam EBM Center of Excellence (CoE). Such centers are popping up increasingly around the world, and for good reason — they offer a sort of ‘one-stop-shop’ approach to a focused manufacturing process.

The new Arcam site is set to house up to 500 employees within its 15,000 square meters. Located in a business park in Härryda municipality, near Gothenburg, the center is said to triple the size of the Mölndal facility, Arcam’s previous site. Significantly larger and set up for a significant workforce, the new CoE is well placed to do some significant business.

Additive Manufacturing Centers of Excellence

The CoE will house facilities for production as well as R&D and training activities and support functions. This is the one-stop-shop benefit: it’s all under the same roof.

As we saw recently in Barcelona with HP’s newest CoE, and in Israel with XJet’s new facility, having access to multiple layers of activity within a single site is a great benefit to a company — and to its customers. 

Each of these companies, including Arcam EBM, is housing new operations in the country where they began their 3D printing activity in the first place. This enhances access to all levels of in-house expertise, though all are very active globally. And, in Arcam’s case, now officially with a US headquarters since its acquisition some years ago by GE Additive.

Lean Manufacturing

The focus for the new Arcam EBM CoE is on lean manufacturing.

Parent GE Additive is keen to emphasize lean manufacturing practices. Wikipedia provides a quick helpful overview of just what this means:

“Lean manufacturing or lean production is a systematic method originating in the Japanese manufacturing industry for the minimization of waste (無駄 muda) within a manufacturing system without sacrificing productivity, which can cause problems. Lean also takes into account waste created through overburden (無理 muri) and unevenness in work loads (斑 mura). Working from the perspective of the client who consumes a product or service, ‘value’ is any action or process that a customer would be willing to pay for.

Lean manufacturing attempts to make obvious what adds value, through reducing everything else (because it is not adding value).”

The company also notes that the CoE is “a dynamic, sustainable workplace,” though no details yet on what that ‘sustainability’ means have yet surfaced.

This often means — as we saw in Barcelona — attention paid in the building of the site to environmentally friendly practices (for example, a photovoltaic roof and recycled water systems). Coinciding with lean manufacturing as outlined above, this also encompasses practices to reduce waste which, helpfully, is also in focus anyway due to the nature of additive manufacturing (as compared with subtractive techniques that inherently produce more waste material during production).

“The Arcam EBM team in Gothenburg is energized to be in its new home — a dynamic, sustainable workplace — in a great location. We will harness that energy and continue to research, innovate and drive EBM technology further,” said Karl Lindblom, General Manager, GE Additive Arcam EBM.

“Throughout, we have benefitted immensely from GE’s experience and know-how in applying Lean manufacturing. Customers joining our annual user group meeting next month will be the first to see our Center of Excellence — which we hope will become a focal point for the entire additive industry.”

Finally, the announcement of the new CoE indicates that it “with an increased manufacturing footprint and focus on R&D will meet that demand and maps to GE Additive’s continued investment in its facilities in the United States and Europe.”

It sounds rather like this next step from GE Additive is by no means the last we’ll be hearing of expansion of their R&D and production efforts.

Via GE Additive

Read More

Page 3 of 46112345